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As you remember from high school science class, colors have different light wave lengths (Oh, you played hooky that day?)

FAQ 1

It took a few black eyes, a few fucking vases to the back of the head

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1 h prior to the assay time point, the medium was exchanged to the Seahorse XF Base Medium (Agilent Technologies) with 1 mM sodium pyruvate, 2 mM l-glutamine and 10 mM d-(+)-glucose (all Sigma-Aldrich), pH 7.4 and incubated at 37 C without CO 2