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Male fertility challenges affect many couples who are trying to conceive, with up to half of infertility cases attributed to male factors alone

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Cleavage of the FRET peptide by MMP-2 and MMP-9 alters the fluorescence of 5-FAM which can be quantified at excitation/emission of 490 nm/520 nm using the BMG Fluostar Optima multimode plate reader (NY, USA)