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The construct was transformed into MAX Efficiency DH5 competent cells (ThermoFisher)

Vitamin B-12 Vegetarians and vegans are more prone to B-12 deficiencies because the nutrient primarily occurs in animal products

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According to the number and location of the catalytic cysteines, Prxs are divided into three classes: the typical 2-Cys, the atypical 2-Cys and the 1-Cys [46]