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Changes in gene expression levels of glutamate-cysteine ligase (GCL), glutathione peroxidase (GPx1), glutathione reductase (GR) and glutathione synthetase (GS) genes were analyzed by real-time qPCR on ABI PRISM 7500 (Applied Biosystems)

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Differences in findings could be attributed to the fact that participants were from different geographic locations, which are known to influence gut microbiota composition through factors such as diet, environment, and lifestyle-potentially contributing to the observed variation