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Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally

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The first tube contained EDTA as an anticoagulant for complete blood count analysis, while the second tube did not contain any anticoagulant and was used for obtaining serum