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The peaks after injection and troughs before the next dose stay within a relatively narrow range

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Based on previous LC-MS/MS measurements used for establishing the LC-MS/MS response curve of CNCbl for the native SIDA method [7], a reverse LC-MS/MS response curve was obtained by plotting peak area ratios [ A ([ 15 N 13 ]-CNCbl)/ A (CNCbl)] against the molar ratios [n([ 15 N 13 ]-CNCbl)/n(CNCbl)] for the quantification of [ 15 N 13 ]-CNCbl