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Liver-specific overexpression of SIRT3 enhances oxidative metabolism, but does not impact metabolic defects induced by high fat feeding in mice

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The pooled calibrator was serially diluted in the extraction solution and used to create a calibration curve (100% (pooled calibrator), 75%, 60%, 40%, 25%, 10%) for relative quantitation

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Blood glucose values typically begin to rise within days to weeks after stopping tirzepatide, though HbA1c changes will take longer to appear since this test reflects average blood sugar over 2-3 months