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Differentiated cells were incubated in media with 1 mg/ml ICG for 1 hour at 37 C, washed with PBS, and imaged using a phase-contrast microscope

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Folate, vitamin B6, and vitamin B12 deficiencies are due to poor dietary intakes, illness, genetic defects that result in impaired absorption or transport, or a combination of these factors.9 Although clinical deficiencies of these vitamins are uncommon in the United States and are usually limited to individuals with genetic mutations, low or marginal deficiencies have been reported, particularly among nonwhite and elderly populations, and women of childbearing age