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Inscopix, USA) implantation, a miniature fluorescence microscope (Inscopix) was mounted onto the GRIN lens base plate

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Also, preincubation of 5M BAPTA-AM, a cell permeant chelator, reduced the uptake of PI induced by ATP to 24% of the plated cells (Fig 7D and 7I) suggesting the involvement of intracellular calcium in this phenomenon

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Leave for 15-20 minutes and remove the sheet