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Mild fluid retention and temporary joint stiffness may also occur as growth hormone levels begin to elevate

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European Journal of Endocrinology (1998) 7

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In addition, the Salkowski technique and different variants have been reported in the detection of IAA at low concentrations (0.5 to 20 g mL -1 ) or higher concentrations (5 to 200 g mL -1 ) ( -1 when no L-tryptophan is added to the medium, and it cannot be detected at 10 g mL -1 when L-tryptophan is supplemented (5 mM) due to a high background absorbance