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PMID: 11045606 Mayfield CK, Bolia IK, Feingold CL, Lin EH et al

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After 24 hpi, each well was washed, fixed with 4% Histofix (Roth), mounted with ProLong Gold antifade mountant (Thermo Fisher Scientific), and visualized at 64 magnification with an Axio Observer microscope (Carl Zeiss Microscopy) using bright field and fluorescence channels (596/621 nm)