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Intracellular activation of nitrofurantoin yields a radical anion that subsequently initiates Nrf2-dependent induction of glutamate-cysteine ligase and consequently a rise in glutathione

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and de novo synthesis of GSH by cancer cells occurs as follows: GSH is first exported from the cell of origin via transporters known as Multiresistance Drug Proteins (MRPs), which belongs to the ATP binding cassette (ABC)s transporter family and is well-known player in cancer resistance mechanisms (33)