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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

Normally, BCAAs (e.g., valine, leucine and isoleucine) can be converted to their corresponding branched-chain keto acids that in turn can be converted to branched-chain CoA, which can also bind carnitine to form branched-chain acylcarnitines (BCACs), such as propionylcarnitine (C3-carnitine), succinylcarnitine (C4DC-carnitine) and isovalerylcarnitine (isoC5-carnitine), which are involved in the energy production process of cells [10]

High dose androgen suppresses natural killer cytotoxicity of castration-resistant prostate cancer cells via altering AR/circFKBP5/miRNA-513a-5p/PD-L1 signals

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