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We then performed the same experiment without adding H 2 O 2 or the spin trap, relying on radical-dependent and O 2 -dependent H 2 O 2 formation, similarly to Amplex Red autoxidation to resorufin in the presence of horseradish peroxidase (HRP) 24 (Extended Data Fig

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397,398 Research has shown that under oxidative stress conditions, mTOR can undergo oxidation at Cys1483, forming intermolecular disulfide bonds, thereby inhibiting cardiomyocyte survival and mitochondrial function

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