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In addition, we carried out similar experiments in which cells were pretreated with ITP at concentrations of 5, 10, and 20 M or insulin at concentrations of 2, 10, 50, 250, and 1250 nM, and then 5 M FITC-ITP was added, and other steps were performed as above

Zhou, Y

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However, alternatives should be used wherever possible (see below)

That complementarity is the rationale behind combination research designs and pre-combined research blends