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The proteins were digested for 2 h at room temperature using 0.5 g Lys-C (Wako Chemicals) and for 16 h at 37 C using 1 g trypsin (Promega), eluted by centrifugation, acidified with TFA and stored at 20 C

Chronic inflammation induces telomere dysfunction and accelerates ageing in mice

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19 In addition, our study also substantiated that pain intensity during TACE was highly correlated with pain intensity 4, 8, and 24 h after TACE, which indicated that achieving excellent pain control during the TACE procedure would lead to lower postoperative pain

Cancer gene mutation frequencies for the U.S