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Peptide bonds are cleaved by proteolytic enzymes in the GI tract within minutes of ingestion

Factors such as trauma, nutritional deficiencies, irritable bowel disorders, leaky gut, microbiome dysbiosis, and immune dysregulation can all contribute to the generation of ROS, leading to tissue damage and ulcer formation

This purification protocol allows a more efficient use of monomeric Fabs compared to Driscoll et al., which requires a 5-fold Fab excess in the second coupling step 34

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Our goal has been to do enough proof-of-principle that people will accept that stapled peptides can get into cells and act on important targets