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however, mitochondrial NAD/NADH ratios are maintained at much lower levels

Cells were incubated at 37 C for up to 5 minutes before aspirating the dissociation solution followed by addition of wash medium (N2B27) supplemented with 10 M ROCK inhibitor, 33 g /mL DNase I (Worthington), and 30 M actinomycin D

Specifically, when HbA 1c was measured in mmol/mol, semaglutide 2.0 mg showed a marginally more pronounced effect compared with placebo than tirzepatide 5 mg, whereas this trend was reversed when analysing HbA 1c in percentage units