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When paired with vitamin C, the liposomal delivery system ensures that both nutrients are available to the cells at the same time
Quantification of lipogenesis gene expression To quantify gene expression, we used the Pure Link RNA Mini kit (12183025, Invitrogen, USA), Hilden, Germany) to extract total RNA from untreated and treated HepG2 cells and used High-Capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA, USA) and 2 g total RNA to prepare cDNA
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Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance