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It occurs exclusively in the cytosol, where glutamate cysteine ligase (GCL) and glutathione synthetase (GS) perform their function

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However, oral supplementation of L-arginine, the precursor for NO synthesis, has been shown to be largely ineffective at increasing NO synthesis and/or bioavailability for several reasons, which makes L-citrulline much more ideal for boosting levels of L-arginine and subsequently NO

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