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By contrast, abdominal aorta blood pressure values were 100120 mm Hg at the level of the bifurcation ( ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time

Standalone supplement

Once reaching near-confluence, culture medium was aspirated and cells were washed once with PBS, followed by a 20 min incubation with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei

To evaluate the therapeutic effect of NHPN-1010 on tinnitus, PPI tests were repeated 8 wks after NHPN-1010 or saline treatment were initiated (corresponding to 12 wks after noise trauma) in the 36 rats that had exhibited behavioral evidence of tinnitus at 4 wks post-exposure

Hansen, K