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The SCFAs acetate (C2), propionate (C3), and butyrate (C4), produced by various microbial species in the gut are the most prevalent anions or metabolites in the gut which have a substantial role in psychiatric diseases (68, 69), in both direct and indirect ways (67)
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Pellets were resuspended in 1 ml lysis buffer [50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% (v/v) Triton-X, complete Protease inhibitor cocktail (Roche)], frozen in liquid nitrogen, and pulverized with mortar and pestle for 10 minutes
Restarting the medication sometimes isn't an easy task, said Dr