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They effectively block acetylcholine (Ach) binding and overstimulate cells at synaptic junctions, obstructing nerve impulse propagation and leading to cell and/or individual paralysis and death (Almeida et al

DM: Writing original draft, Investigation, Formal analysis, Data curation

2019;241(1):R1R33

Subsequently, the cells were stained with the Hoechst 33342 nuclear dye (Thermo Fisher Scientific, Inc.) at a 1:500 dilution in PBS at room temperature for 10 min, washed with PBS, and visualized using a BZ-X700 (Keyence Corporation)

Orlistat, which works by reducing fat absorption rather than suppressing appetite, may carry different risk profiles, though it can cause gastrointestinal side effects