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Isotopically labeled metabolic substrates were used to monitor generation of 3 H 2 O from glycolysis or beta oxidation and 14 CO 2 from the pentose phosphate pathway, glutamine utilization, or TCA cycle

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Once oxidized during ROS detoxification (most notably through glutathione peroxidasemediated reduction of hydrogen peroxide), glutathione must be regenerated from its oxidized form (GSSG) by glutathione reductase, a process dependent on a continuous supply of NADPH from the pentose phosphate pathway (3)