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Tissues were dissected, post-fixed for 3 h in 4% PFA at room temperature 1820 2 C cryoprotected in 30% (w/v) sucrose in Tris-Azide buffer (40 mM Tris, 10 mM phosphate, 0.7% (w/v) NaCl, 0.05% (w/v) NaN 3 ) at 4 C, then embedded in optimal cutting temperature (OCT) compound, rapidly frozen in isopentane on dry ice, and stored at 80 C until use

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(2) the slightly acidic pH (4.57.0) is compatible with most peptide structures

assessed the involvement of PLA 2 and SMase in the molecular pathways leading to oxidative stress-induced eryptosis in workers exposed to lead

Vitamin C : Mechanism : Vitamin C acts as an antioxidant and plays a vital role in collagen synthesis