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It is synthesised in the cytosol by glutamate-cysteine ligase (EC 6.3.2.2) and glutathione synthetase, with the first, rate-limiting step feedback-inhibited by glutathione itself and intracellular concentrations typically 1 to 8 mM (Griffith, Free Radical Biology and Medicine, 1999)
Grandjean EM, Berthet P, Ruffmann R, Leuenberger P
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75 Amino acid metabolism Amino acids are the primary substrates for protein biosynthesis, and recent evidence emphasizes the critical role of amino acid availability and metabolism in the regulation of antitumor immunity
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