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Activity-based DUB probe labeling in brain tissues Mouse and killifish brains were lysed in DUB buffer consisting of 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.1% NP-40, 5% CHAPS, 5 mM MgCl, 5 mM -mercaptoethanol, and 10% glycerol

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doi:10.1056/NEJMoa2200433

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Hold for 30-90 seconds before swallowing

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