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J Biol Chem 293(23):88748885
The reconstituted solution will not be colourless: expect a blue to blue-violet tint from the Cu(II) centre of the GHK-Cu component, and treat a colourless or green-tinged solution as evidence the copper complex has been disrupted rather than merely diluted, with visible absorption around 500 to 600 nm as the standard check
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