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Sections (4 m thick) were prepared and stained with hematoxylin and eosin (H&E) or immunostained with primary antibodies: rabbit monoclonal antiTg (ab156008, Abcam, Cambridge, UK, dilution of 1:250), rabbit monoclonal antiKi67 (ab66155, Abcam, dilution of 1:100), rabbit polyclonal antitumor suppressor P53binding protein 1 (53BP1) (A300272A, Bethyl Laboratories, Montgomery, TX, USA, dilution of 1:200), rabbit monoclonal antiMIEAP (ab180154, Abcam, dilution of 1:400), and rabbit polyclonal antiTOMM20 (translocase of outer mitochondrial membrane 20) (118021AP, Proteintech, Tokyo, Japan, dilution of 1:250)

You should store it at room temperature between 59F and 85F

App-based plotters offer better day-to-day tracking integration

Shorter shelf life means tighter adherence to storage protocols

A key target is the Kv7 (KCNQ) family of voltage-gated potassium channels