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[DOI] [PubMed] [Google Scholar] 113.Wang X.Y., Qu M., Duan R., Shi D., Jin L., Gao J., Wood J.D., Li J., Wang G.D

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Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls

CNS injury-induced astrocyte activation has strengthened the view that many neurodegenerative diseases involve non-cell-autonomous mechanisms, where glial cells, especially astrocytes, actively shape disease progression