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It measures free radical scavenging capacity by accepting electrons or hydrogen atom, converting the DPPH radical into a stable diamagnetic molecule (Baliyan et al., 2022).The assay operates on the principle of measuring the reduction of the DPPH radical, a stable radical with a deep purple color that exhibits strong absorption at 515520 nm(Sridhar & Charles, 2019).When antioxidants are present, they donate hydrogen atom to the DPPH radical, causing a color change from purple to yellow, which is quantified spectrophotometrically at 517 nm (Arsul et al., 2022
The use of water as a solvent under subcritical conditions also necessitates additional steps, such as evaporation or dehydration, to remove water from the extracts, increasing processing complexity (66)
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