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Abstract Glucagon-like peptide-1 receptor agonists (GLP-1RAs), initially developed for diabetes and obesity, exhibit pleiotropic effectsincluding anti-inflammatory, antioxidant, and neuroprotective propertiesthat position them as transformative candidates for ocular therapeutics

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Site-directed mutagenesis of GLP-1R cholesterol binding sites Human SNAP/FLAG-tagged GLP-1R (SNAP/FLAG-hGLP-1R) (Cisbio) was used as the template to generate GLP-1R cholesterol binding mutants for screening