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After washing with HBSS to remove Percoll, cells were resuspended in DMEM/F12 with 10% FBS and plated on collagen-coated plates and cultured at 37 C in a 5% CO 2 incubator

Primary research: Sleep regulation, circadian rhythm, stress response Mechanism: Modulates sleep spindle activity and interacts with multiple neurotransmitter systems including GABA, serotonin, and glutamate Regulatory status: Not FDA-approved

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Chronic high-dose morphine treatment promotes SH-SY5Y cell apoptosis via c-jun N-terminal kinase-mediated activation of mitochondria-dependent pathway