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Common analytical methods Total glutathione and the GSH:GSSG ratio are typically measured by enzymatic recycling assays (using glutathione reductase and DTNB, the Ellmans reagent), by high-performance liquid chromatography (HPLC) with various detection methods, or by mass spectrometry

doi: 10.1016/J.PHRS.2004.06.002 75 LeeDKimKHLeeJHwangGSLeeHLHahmDHet al

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[63] first reported the existence of G protein-coupled receptor (GPCR) GLP-1R in human hepatocytes in 2010, and provided evidence to prove the direct effects of GLP-1 protein on cells