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Veillard NR, Kwak B, Pelli G, Mulhaupt F, James RW, Proudfoot AE, Mach F

After primary antibody incubation, slices were then washed with PBS three times and incubated with secondary antibody donkey anti-mouse IgG (H + L) Alexa Fluor 488 (1:1000, Thermofisher Scientific, A-21202) in blocking solution for 90 min

These hydrolyses act as mononuclear iron enzymes

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Or, more specifically, how one of these enzymes starts to fail with age or persistent illness