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Cell culture Murine HL-1 and H9C2 cardiomyocytes were purchased from the Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, and were cultured in high-glucose DMEM containing 10% FBS and penicillin/streptomycin at 37C in a 5% CO 2 incubator

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f , HEK293T cells were transfected with increasing amounts of mouse Tmeff1 expression plasmid and infected with HSV-1-GFP

As a result, the integrative framework proposed in this review, while conceptually useful, may not fully capture the temporal and individual variability observed in real-world settings

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