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To detect Ki-67 binding, a biotinylated goat anti-rabbit secondary antibody was used at 1:250 dilution for 30 minutes, followed by the ABC reagent (Vector, AK-5000) for 5 minutes

Rolipram-induced improvement of cognitive function correlates with changes in hippocampal CREB phosphorylation, BDNF and Arc protein levels

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In addition, due to its protein nature it is inactivated in the gastrointestinal tract, not being absorbed (practically null oral bioavailability), which hinders or prevents the passage into plasma of the infant from ingested breast milk (Serrano 2015), except in premature infants and during the immediate neonatal period, in which there may be greater intestinal permeability

Could be useful evidence to show GP of symptoms returning before next injection