Initial processing involved removing experimental barcodes (iCount version 2.0.0, de-multiplex function followed by read alignment with STAR 131 against the human reference genome (hg38, GRCh38.p13 primary assembly, GENCODE version 39), allowing up to two mismatches
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Depending on the nature of this final cleavage, the procedure can be modified to give a C-terminal acyl thiocyanate peptide product which automatically rearranges to a thiohydantoin incorporating the penultimate C-terminal unit