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Titration of GRX1-roGFP2 and roGFP2 was done with DTT, which has a more negative reduction potential than lipoic acid ( E DTT = 323 mV, Shaked et al., Plant material and growth conditions Heterozygous Arabidopsis rml1 mutants were selected by genotyping for the mutant allele and exploiting the fact that the point mutation (Vernoux et al., rml1 seedlings were selected for further propagation