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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)

After the 56-week treatment period, BMI standard deviation scores (BMI SDS) decreased by a mean of 0.23 in the liraglutide group, with no change in the placebo group

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It is sold as a research chemical NNMT inhibition has broad implications : NNMT is involved in multiple metabolic pathways beyond NAD+, including methylation balance, polyamine metabolism, and one-carbon metabolism