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The restricted use of mucoadhesive particle systems in the clinic teaches us an important lesson for future inhaled drug development: preclinical and clinical studies should include various cell lines, tissues and disease models that represent the complexity of human lung biology (Fig

Western blotting Protein samples were separated by electrophoresis and transferred to a nitrocellulose membrane (Millipore, USA), blocked with 5% non-fat milk for 2 h, and then incubated overnight at 4 C with primary antibodies specific for PCNA (WANLEIBIO), cyclin A (WANLEIBIO), CDK2 (Bioss), TGF- (Bioss), Caspase 3 (Bioss), Bax (Boster), Bcl-2 (Bioss), MMP2 (Bioss), MMP9 (Bioss), AMPK1 (Bioss), HSP47 (Boster), NLRP3 (WANLEIBIO), Caspase 1 (WANLEIBIO), IL-18 (Affinty), and IL-1 (Bioss)

These are not randomized controlled trials

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