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To improve the selectivity, in this work, we design and synthesize a malononitrile-functionalized TPE derivative, TPE-DCV, which can undergo addition reaction with thiol group to the alkene bond, leading to the change in its fluorescence in the system (Figure 1)

Des tudes de la structure de leurs gnes suggrent que ces diffrents enzymes drivent d'un anctre commun (Warden et al., 1993)

10.1021/bi00838a031 221 GriffithsJ

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The hydroxyl group of the Tyr/Ser residue forms a hydrogen bond with the sulfhydryl group of GSH to promote the formation and stabilization of the thiolate anion