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Early efforts to understand the regulation of glutathione biosynthesis in plants focused on substrate availability, feedback inhibition, and transcriptional control with more recent studies investigating post-translational modifications (Yi et al., 2010a,b)

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Gating residues involved in the occluded-to-inward conformational transition of OCTN2 Alternating-access transport by secondary active transporters requires the coordination of gates that enclose or preclude access to the substrate-binding site from either side of the membrane 51

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