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After blocking in 5% milk in Tris-buffered saline buffer (TBST), membranes were exposed to primary antibodies against GLP-1R (# bs-1559R, Bioss Antibodies, Woburn, MA, USA, 1:1000 dilution in 5% BSA in TBST, incubated overnight at 4 C) or -actin (#A1978, Sigma-Aldrich, St

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