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Although some experimental studies have reported reduced cavernous nerve fiber density and impaired neurotransmitter release in varicocele-related settings, much of the current evidence remains derived from non-varicocele nerve injury models or broader ED research (111, 155)

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The mice were housed in groups of nine animals during the study, under controlled environment (22 1 C) with a relative humidity (50 10%) and a normal 12 h light cycle (at 8:00 pm lights off)