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Weve explored the critical distinction between unopened pens, which always require refrigeration, and in-use pens, which offer the flexibility of either refrigerated or room temperature storage for up to 56 days

Instead, lingering issues like tissue damage or immune dysregulation remain

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Metaphors, such as referring to GLP-1 medications as training wheels for lifestyle changes, were also cited as helpful communication strategies

Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)