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Cells were then washed and resuspended in 200 cL flow cytometry staining buffer and analyzed using FACSAria III cell sorter (BD Biosciences, USA)

Amino N, Kuro R, Tanizawa O, Tanaka F, Hayashi C, Kotani K, Kawashima M, Miyai K, Kumahara Y

The recommended frequency is usually 1-2 sessions per week for the first month to build up your glutathione levels

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