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After 1 mM CaCl 2 was added for Tse3-Tsi3 protein interaction, the supernatant was then loaded to the CNBr-activated Sepharose resin (GE Healthcare) conjugated with Tse3 protein by gravity flow

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The second PCA component, loading on glutathione and glutamate scores in the ACC, was therefore our first dependent variable

This unique cross-talk between mitochondria and nuclear DNA positions MOTS-c as a regulator of metabolic adaptation , a crucial function that tends to decline with age