The principal component analysis (PCA) effectively and distinctly separated the 2 groups ( x axis in Figure 1B), suggesting that the absence of protein 4.2 caused a significant change in the overall metabolite profile compared with healthy erythrocytes (Figure 1B)
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2.3.2 Automatic biochemical analysis and testing A fully automated biochemical analyser (AU5800, Beckman Coulter, USA) was utilised to ascertain serum biochemical indices, encompassing the following: Liver function indices: alanine transaminase (ALT), aspartate transaminase (AST), and gamma-glutamyltransferase (GGT)
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The involvement of particular amino acids, like arginine, within the CCP is crucial in the targeting procedure as the positively charged guanidine group in arginine can bind to negatively charged components of bacteria cell membranes, helping in the entry and uptake of NPs [213, 214]
