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A total of 1 mL 1 M KCl was added to the samples, followed by vortexing and centrifugation as described, and the upper phase was subsequently discarded

In lupus-prone females, reduced abundance of Erysipelotrichaceae and Bifidobacterium, with increased Lachnospiraceae and Bacteroidetes S247 abundance, has also been reported ( These findings suggest that microbiological dysbioses specific to sex, more precisely the increased prevalence of Lachnospiraceae in females, may account for the earlier onset and greater severity of lupus symptoms

Hence, an increase in the GSH to GSSG ratio favors protein deglutathionylation

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PMID 16307756